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HPLC systems are currently used in a wide variety of fields. Higher reliability in analysis data and higher efficiency in total analysis workflow are required for faster development of new drugs, for better food safety, and for meeting higher standards in environmental regulations. Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to semi-micro range and injectors with low sample carryover. Prominence is a network-ready HPLC system that meets the demands of today’s advanced users. Prominence features the world's first Web control, fastest sample injection, and highest detection sensitivity performance…functions that surpass current HPLC technology.
High Speed Injection
Only 10 seconds (for 10uL injection) are required for sample injection. High-speed vertical motion of the needle enables ultra-high speed sample processing, which was considered impossible with conventional auto-samplers. Using in combination with a high-speed-separation column makes an analysis cycle of less than one minute a reality.
Large Number of Samples Processing by Rack Changer
SIL-20A/C standard autosampler handles up to 768 sample (using 386-well microplates, 175 samples with 1mL vials). A rack changer is an optional product that can be used to change the microplates in the auto-sampler's racks and thereby facilitate serial analysis. Up to 12 plates can be mounted in the rack changer.
Combining the harmonize functions for each unit makes it possible to fully automate all the processes involved in analysis workflow, from startup to shutdown. Simply set the mobile phase and sample and start the system. The system will recognized the operating status and execute analysis. This significantly reduces the time the user must devote to analysis at startup.
Automated analysis procedure includes: Starting and stopping of system operation at specified times, Replacement of mobile phase (purge), Automatic judgment of baseline stabilization, Rinsing and cooling of column, sample injection.
Self-diagnostics (Expert Function)
The system is equipped with a function that continuously monitors the operating status and, if there is a problem, provides notification and tries to resolve it. For example, if the pressure falls below a certain value during serial analysis, the function detects the problem, purges the mobile phase, and then resumes analysis after restoring normal status. The reliability of operating performance is also increased with a variety of diagnostic functions.
Automated check items include:Remaining amount of mobile phase, Noise/drift check, Vial detection.
The Prominence system also incorporates consideration of validation. IQ/OQ at the time of installation, operational qualification for each unit in periodic inspections, and management of maintenance information can all be executed with ease. This means that inspections and modifications can be performed quickly and simply, significantly reducing the validation cost. Also, using an optional CMD (Column Management Device) makes it possible to recognize the column name and serial number, and to leave a record of information, such as the amount of mobile phase that has pumped and the number of sample injections.
Automated operational-qualification items include; Accuracy of solvent-delivery flow rate, Solvent-delivery pulsation, Lamp energy, Wavelength accuracy with built-in low pressure mercury lamp.
System Control Using a Web Browser
HPLC control through Internet Explorer
Use any computer with Microsoft Internet Explorer 6.0 to control and monitor the Prominence HPLC system. Connection to the CBM-20A with a static IP address requires no special software to control and monitor HPLC systems on the network from the client PC. Easily check the status of networked HPLC systems, or monitor consumable parts for increased work efficiency.
High Sensitivity and Linearity
The SPD-20A UV-VIS detectors achieve the highest level of detection sensitivity 0.5 x 10(-5)AU and linearity (2.5AU) by electronic noise elimination and a temperature-controlled flow cell. This performance is ideal for everyday needs and especially suitable for high-sensitivity analyses such as impurity quantitation. The SPD-M20A Photo-diode Array detector offers a sensitivity level that, at 0.6 x 10(-5) AU, is comparable to that of UV-VIs detectors.
Minimized Sample Carryover
Adsorption of sample constituents has been reduced to an absolute minimum by using a special processing technology for the sampling needle (patent pending) and rethinking the structure of the needle seal and the materials used in flow-line parts. As a result, there is hardly any sample carryover. Greater accuracy has been attained by incorporating a high-performance sampling device that can measure our the samples with high precision.
The SPD-20A/20AV and SPD-M20A are equipped with a temperature-controlled flow cell as a standard feature. This helps increase the baseline stability and the analysis reliability.
Higher Flow Rate Accuracy
The flow-rate accuracy and precision in the micro-flow-rate region have been improved thanks to the adoption of a new type of check valve and modifications to the solvent-delivery control method. This makes the Prominence an ideal front-end for high-sensitivity analysis with LC-MS.
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